This signifies that option-clicking a layer in the legend of a graph will delete that layer from the graph. If you hold down the option key, you'll see that the cursor changes from a vertical reordering tool into a trash can. Dragging the order of the layers will cause the graph to be redrawn, so you can quickly see which layers you want to feature in the top positions. I would like to change to : FL1-FITC-CD8 and FL2-PE-CD4. Set up of file name and saving location preferences iii. I mis-labelled one sample: For example FL1-FITC-CD45 and FL2-PE-CD8. Load some data in FlowJo and in the workspace Right-click on a sample Go to Sample Info (or. The Cytometer Window: Its uses include i. FIL Name of the data file containing the data set. Use Short File Name: Uses the file name as seen by the operating system for sample names Use Full Path and File: Used when resolving. Sample Display Naming FCS Default: Uses the FCS standard identification keyword as the display name for samples in the workspace. The Workspace: Used to create a sample list and to apply attributes (e.g., antibody/fluorochrome labels) to the samples listed 2. Select the Workspace button in the Preferences tool to open the Workspace preferences. ![]() Because events in front can obscure others, you may want to have the smaller populations on top of the larger ones, though this depends greatly on the context of your analysis. 3.1.1 FlowJo Collectors’ Edition has three main components: 1. Moving a graph's name to the top of thelist moves the graph to the top of the overlay stack. The order of the lines in the legend mirrors the order of the graphs displayed. This tools allows you to reorder the legend. If you move the mouse over the text description of the layers, you'll see the cursor become a pair of bars with vertical arrows sticking out of it. Clicking the mouse on the outside edge of the legend lets you move or resize the legend. When the mouse passes over an edge of the legend, the cursor will become a hand. Drop the second graph (or more, if you had multiple nodes selected in the workspace) on top of the original, and an overlay is automatically created. As the mouse moves into the original graph item, you'll see that it highlights its borders to signify that it will accept the contents of the drag. Then drag a second on top of the first one. To create an overlay, drag a graph into the Layout Editor. ![]() Once an overlay has been defined, the layout editor can create the same graph for many different samples or sets of samples, by iterating over a group in the workspace. Within the Layout Editor you can edit the color and order of the items, to customize the look of the overlay graph that best highlights your data. Overlays may be created on either univariate (right) or bivariate displays (below). This makes it easy to compare the data, using differing colors to show multiple populations at the same time. Often it is useful to plot two or more data sets or population on the same axes.
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